Analysis of Factors Affecting Microscopic Displacement Efficiency in CO2 Floods

اختصاصی از حامی فایل Analysis of Factors Affecting Microscopic Displacement Efficiency in CO2 Floods دانلود با لینک مستقیم و پر سرعت .

مقاله SPE شماره 70022

دانلود پاورپوینت تحلیل درونی و برونی SWOT analysis

اختصاصی از حامی فایل دانلود پاورپوینت تحلیل درونی و برونی SWOT analysis دانلود با لینک مستقیم و پر سرعت .

موضوع: دانلود پاورپوینت تحلیل درونی و برونی  SWOT analysis

تعداد اسلاید: 63

Analysis of sulfonamides in environmental water samples based on magnetic mixed hemimicelles solid-phase extraction coupled wit

اختصاصی از حامی فایل Analysis of sulfonamides in environmental water samples based on magnetic mixed hemimicelles solid-phase extraction coupled with HPLC–UV detection دانلود با لینک مستقیم و پر سرعت .

Steady-state rolling analysis of a tire

اختصاصی از حامی فایل Steady-state rolling analysis of a tire دانلود با لینک مستقیم و پر سرعت .

اختصاصی از حامی فایل Substrate-binding specificity of chitinase and chitosanase as revealed by active-site architecture analysis دانلود با لینک مستقیم و پر سرعت .

Substrate-binding specificity of chitinase and chitosanase as revealed by active-site architecture analysis

Abstract

Chitinases and chitosanases, referred to as chitinolytic enzymes, are two important categories of glycoside hydrolases (GH) that play a key role in degrading chitin and chitosan, two naturally abundant polysaccharides. Here, we investigate the active site architecture of the major chitosanase (GH8, GH46) and chitinase families (GH18, GH19). Both charged (Glu, His, Arg, Asp) and aromatic amino acids (Tyr, Trp, Phe) are observed with higher frequency within chitinolytic active sites as compared to elsewhere in the enzyme structure, indicating significant roles related to enzyme function. Hydrogen bonds between chitinolytic enzymes and the substrate C2 functional groups, i.e. amino groups and N-acetyl groups, drive substrate recognition, while non-specific CH-π interactions between aromatic residues and substrate mainly contribute to tighter binding and enhanced processivity evident in GH8 and GH18 enzymes. For different families of chitinolytic enzymes, the number, type, and position of substrate atoms bound in the active site vary, resulting in different substrate-binding specificities. The data presented here explain the synergistic action of multiple enzyme families at a molecular level and provide a more reasonable method for functional annotation, which can be further applied towards the practical engineering of chitinases and chitosanases.

Keywords

• Chitinase ,Chitosanase, Active-site architecture, Substrate-binding specificity